Association of semenogelin (SEMG) gene variants in idiopathic male infertility in Chinese-Han population

ABSTRACT

Infertility is known to occur frequently worldwide, and the incidence is continuing to rise in China. It is known that semenogelin (SEMG) protein secreted by human seminal vesicles plays an important role in male reproductive system function. However, an association between alterations in SEMG gene functions and idiopathic male infertility occurrence in Chinese-Han population has not been examined. The aim of this study was thus to investigate the inherent relationship between SEMG gene alterations and idiopathic male infertility using a method of variant genotyping selection and semen quality analysis. A population of 484 males with clinically diagnosed idiopathic male infertility and 246 fertile controls were selected after signing consent forms. Results demonstrated a significantly increased frequency of idiopathic infertility with abnormal semen parameters such as semen volume, sperm concentration, sperm number per ejaculate, and sperm motility in variants carrying the rs2301366 TA genotype. Combined association analysis from target single-nucleotide polymorphisms (SNPs) was selected from the genotype database of unrelated Chinese-Han in Beijing individuals from the Hap Map. SNP array analysis in blood samples in each group was carried out by TaqMan Universal PCR Master Mix and TaqMan SNP Genotyping Assays. In addition, the interaction between SEMG SNPs and binding protein epididymal protease inhibitor (EPPIN) SNPs was determined. Our findings demonstrated that the presence of SEMG SNPs and EPPIN SNPs increased the frequency of idiopathic male infertility in Chinese-Han population. It is proposed that measurement of SEMG SNPs and EPPIN SNPs in carriers may thus be utilized to identify idiopathic male infertility.

KEYWORDS:

• SEMG gene
• EPPIN gene
• genetic variants
• male idiopathic infertility

Additional information

Funding

This study was supported by the National Natural Science Foundation of China (81673203, 81703196, 81803271), Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Young Medical Talents of Science and Technology Enhanced Health Project of Jiangsu Province (QNRC2016174), Medical Development Discipline Project of Wuxi (FZXK007), Major project of Wuxi Commission of Health (Z201812) and Project of Wuxi Science and Technology Bureau (WX18IIAN040, CSZ0N1726).