Investigation of Microbes in the Rhizosphere of Selected Grasses for Rhizoremediation of Hydrocarbon-Contaminated Soils

Abstract

Hydrocarbon-degrading microorganisms (HDM) associated with the rhizosphere of Paspalum vaginatum and Zoysia tenuifolia grown in bioremediated soil were isolated under controlled laboratory conditions. The isolation process was conducted at 30°C and 45°C to isolate mesophilic and thermotolerant microorganisms, respectively, under aerobic conditions. The isolated HDMs were identified using 16S rRNA gene sequencing and fatty acid methyl ester (FAME) analysis. Although differences in the genera of the isolated HDMs occurred between the two grasses, Arthrobacter spp and Bacillus spp were isolated from the rhizosphere of both plants. The efficiency of the isolated microorganisms in degrading a mixture of hydrocarbon compounds (HC) was also assessed. Among the bacterial isolates, Pseudomonas boreopolis was found to be the most effective HC degrader, while the only fungal isolate, Fusarium solani, demonstrated higher degradation rates than most of the bacterial isolates. A mixture of all the microbial isolates demonstrated a high degradation percent of HC. The isolated microorganisms thus appear to work synergistically to degrade efficiently all the tested organic compounds.

Keywords:

• Rhizosphere
• hydrocarbon degrading microorganisms
• Paspalum vaginatum
• Zoysia tenuifolia