Measurement of Immunoglobulins G, A, and M Levels in B‐Lymphocytes Culture

Abstract

Nephelometric immunoassays were developed for human IgG, IgA, and IgM quantitation in B‐lymphocytes culture media. They allowed measurement of immunoglobulin (Ig) levels over a broad range of concentrations with good accuracy and precision. The kinetics of Ig production in B‐lymphocyte cultures was followed and the mean amount of each Ig was determined in six different samples after three days of culture. The nephelometric immunoassays reported here could be used to study, in vitro, the influence of various molecules (inhibitory or amplifying effect) on B‐lymphocytes' functional capacities.

Keywords:

• Immunoglobulin G
• Immunoglobulin A
• Immunoglobulin M
• Lymphocytes culture media
• Immunoassay
• Immunonephelometry