Selective Identification of Vα14i T Cells Using Slide‐Immobilized, CD1d‐Antigen Complexes

Abstract

The ability to correlate changes in antigen‐reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1‐restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1‐restricted lymphocytes in humans. To investigate the feasibility of this technique, we employed mCD1d and α‐galactosylceramide to demonstrate that‐slide immobilized, CD1d‐α‐GalCer complexes capture an NKT cell hybridoma in the presence of a competitor. The success of this scheme represents the first step toward the development of CD1‐antigen arrays that could be used to profile biological samples.

Keywords:

• CD1
• Antigen
• NKT cell
• Array
• Glycolipid
• Hybridoma

Acknowledgments

The authors thank Jennie Sturgis, J. Paul Robinson and the Purdue Analytical Cytology Resource for technical assistance with confocal microscopy. This work was supported by PHS AI46455, CA89026 (R.R.B.), and CA78264 (E.J.T).