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Abstract
A novel monoclonal antibody (MAb) to hippuric acid (HA) was prepared using an HA analog, N‐α‐benzoyl‐lysine, as an immunogen. An enzyme‐linked immunosorbent assay (ELISA) for HA was established using the anti‐HA MAb named HA01BL. When the specificity of the MAb was analyzed by the ELISA system, the MAb was revealed to be less reactive to methylhippuric acids and to be more specific to HA than previously reported polyclonal antibodies. The detection limit of HA by the ELISA was approximately 1 µg/mL. The urinary HA concentration determined by the ELISA system correlated well with that obtained by high performance liquid chromatography.
Acknowledgments
This work was partly supported by a Grant‐in‐Aid for Encouragement of Young Scientists from The Ministry of Education, Science, Sports and Culture of Japan (No. 08770287). The authors thank Miss N. Gotoh and Dr. M. Katsumata for their skillful technical assistance. We are also grateful to Drs. T. Jitsukawa and Q. Li for their helpful discussions.