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Journal of Immunoassay and Immunochemistry Volume 27, 2006 - Issue 3
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Original Articles

Establishment and Application of Enzyme Immunoassay for Saliva Cortisol in Taiwanese Context

Han‐Lin Chen Department of Medical Technology and Institute of Biotechnology, Yuanpei University of Science and Technology, Hsinchu, Taiwan, R.O.C.
,
Yung‐Liang Chen Department of Medical Technology and Institute of Biotechnology, Yuanpei University of Science and Technology, Hsinchu, Taiwan, R.O.C.Correspondence[email protected]
,
Leang‐Shin Wu Department of Animal Science, National Taiwan University, Taipei, Taiwan, R.O.C.
,
Krishna Kaphle Department of Animal Science, National Taiwan University, Taipei, Taiwan, R.O.C.
&
Jen‐Hsou Lin Department of Animal Science, National Taiwan University, Taipei, Taiwan, R.O.C.
Pages 239-249 | Received 08 Jul 2005, Accepted 14 Aug 2005, Published online: 06 Feb 2007
 
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Abstract

Saliva steroid assay is an upcoming area of research, with much potential for growth and progress. Expensive, varying results with commercial kits and the disadvantages of radioimmunoassay have forced researchers to develop their own system of enzyme immunoassay (EIA). A modification from our established EIA system was used to develop a saliva cortisol (F) assay system. The system sensitivity (>90 pg/mL) was checked by various experiments, including comparison of data with a commercial kit obtained from Salimetrics®. The assay system was employed to investigate the saliva F level in a young Taiwanese population, and compared with the total and free serum levels of F.

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