Assessment of Chromogen Suitability in ELISA for the Detection of Anaplasmosis and Trypanosomosis

Abstract

Two different ELISAs were routinely performed in our laboratory to detect bovine trypanosomosis and anaplasmosis. The ELISA test for trypanosomosis involved the adsorption of a soluble fraction of parasites as the antigen; and, the ELISA for anaplasmosis was performed with a purified recombinant protein MSP5r adsorbed to the plate. With the purpose of assessing the merit of ABTS and TMB, we compared the absorbance obtained from positive and negative control sera from both assays. The results obtained, suggest that TMB is more adequate for recombinant antigens and that ABTS is preferred when partially purified antigenic extracts are used in the ELISA test.

Keywords:

• Anaplasmosis
• Tripanosomosis
• TMB
• ABTS
• MSP5
• ELISA

Acknowledgments

We thank Xiomara Ortega for her excellent technical assistance and H. Caballero of Universidad Simón Bolívar for the hyperinmune serum. This research was supported by “Projects G‐98003462 and “Iniciativas del Milenio, Estación Experimental La Iguana” by Fondo Nacional de Ciencia y Tecnología (FONACIT), from Ministerio de Ciencia y Tecnología, Venezuela.