Comparison between Chicken and Rabbit Antibody Based Particle Enhanced Cystatin C Reagents for Immunoturbidimetry

Abstract

We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle‐enhanced immunoassays.

Keywords:

• Chicken antibodies
• IgY
• Rabbit antibodies
• Latex particles
• Particle‐enhanced immunoassays
• Cystatin C

Acknowledgments

This project was supported by the Uppsala University Hospital Research fund.