Establishment of Hybrid Cell Lines Producing Monoclonal Antibodies to a Synthetic Peptide from the E1 Region of the Hepatitis C Virus

Abstract

We aimed at establishing hybridoma cells secreting monoclonal antibodies (mAbs) against E1 synthetic peptide of HCV. BALB/c mice were immunized with HCV E1‐synthetic peptide (GHRMAWDMM) and its spleenocytes were fused with the P3NS1 myeloma cell line. Two highly reactive and specific mAbs (10C7 IgG2b mAb, and 10B2 IgG1 mAb) were generated. The target HCV E1 antigen was identified at ∼38 kDa in serum of infected individuals. A newly developed ELISA detected the target antigen in 90% of sera from HCV RNA infected individuals with a specificity of 84%. So, the generated mAbs may provide promising probes for serodiagnosis of HCV infection.

Keywords:

• Hepatitis C virus
• Hybridoma
• Monoclonal antibody
• Envelope 1
• Antigen
• Diagnosis

Acknowledgments

The authors would like to thank Yasmine S. El‐Abd at NRC for her kind help. The present work is supported in part by Ministry of Scientific Research, Academy of Scientific Research & Technology (Medical Research Council Code: P5‐MED‐030‐01), and National Strategy for Genetic Engineering and Biotechnology Projects, Egypt.