Development of a Storage‐Compatible Microtiter Plate‐Based Technique for Lymphocyte Proliferation

Abstract

The lymphocyte proliferation assay (LPA) is an important tier 1 test to assess non‐specific lymphocyte function, in vitro. However, this assay requires fresh preparation, is time consuming, and labor intensive. Developing a plate coating technique for lymphocyte proliferation that is both stable and storage compatible would be useful to the basic and clinical researcher. In this study, we compared the effects of different mitogen plate coating techniques on lymphocyte proliferation to freshly prepared plates. The results show that plates prepared with complete media and stored at −40°C up to 10 days corresponded well to control plates.

Keywords:

• Lymphocyte
• Proliferation
• Mouse
• Mitogen
• Plate‐coating
• In vitro

ACKNOWLEDGMENT

The authors would like to thank Ms. Shannon Viers for the care and maintenance of the animal colonies.