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Original Articles

Development of an Immunoassay to Measure Progesterone using Printed Biosensors, and its Application to the Assessment of Ovarian Function in the Numbat (Myrmecobius fasciatus)

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Pages 232-243 | Received 10 Apr 2008, Accepted 21 Aug 2008, Published online: 28 Mar 2009
 

Abstract

A biosensor system was developed to measure progesterone levels in the urine of female numbats (Myrmecobius fasciata) as an index of ovarian function. Screen printed sensors were coated with a monoclonal progesterone antibody, and incubated in a mixture of sample/standard and progesterone-3-CMO-horseradish peroxidase (HRP). The difference in potential between the working and reference electrode was measured, after exposure to an HRP substrate. EIA and biosensor standard curves showed parallelism, and the biosensor gave values similar (r = 0.83) to the conventional EIA. Progesterone concentrations at different stages of the oestrus cycle were not significantly different to those obtained by EIA.

ACKNOWLEDGMENT

The role of Vicki Power, Cathy Lambert and the keeping staff at Perth Zoo in maintaining the numbats and collecting the urine is greatly appreciated.

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