COMPARISON OF SPEEDY PCR-SSP METHOD AND SEROLOGICAL TYPING OF HLA-A24 FOR JAPANESE CANCER PATIENTS

Abstract

Human leukocyte antigen (HLA) typing is essential to carry out HLA-class I restricted antigenic peptide-based cancer immunotherapy. To establish a one-step polymerase chain reaction–sequence-specific primer (PCR-SSP) method, we designed two novel HLA-A24-specific primer sets and determined the optimal conditions for specific amplification. Then, we performed HLA-A24 typing of two healthy donors’ and 17 cancer patients’ peripheral blood with serological typing and PCR-SSP typing. Eleven of the 19 cases were determined HLA-A24-positive by the PCR-SSP method precisely; however, five cases showed false positive with serological analysis. Thus, for HLA-A24 typing in the Japanese population, the PCR-SSP method is faster and more accurate than serological typing.

Keywords:

• HLA-A24
• HLA-typing
• monoclonal antibody
• PCR-SSP

ACKNOWLEDGMENTS

The authors thank Drs. P. G. Coulie and B. J. van den Eynde for kindly providing cell lines. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. All authors have declared that there are no financial conflicts of interest in regard to this work.

Notes

n.d.: not detected.