Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method

Abstract

IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.

Keywords:

• cerebrospinal fluid
• immunoglobulins
• kappa/lambda ratio
• ELISA
• isoectric focusing

Acknowledgments

We would like to thank The Binding Site Ltd. (Birmingham, UK) for the kind donation of Hevylite IgG kappa and IgG lambda antibodies for research use. We are also greatly indebted to Dr. P. Hradílek (MS Centre, Clinic of Neurology, University Hospital Ostrava) and Dr. Alexandra Lochmenová (Immunology and Allergology Laboratory, Public Health Institute, Ostrava) for fruitful discussions and to Dr. V. Procházka, Dr. K. Vítková and Dr. J. Jurcíková (Dept. of Science and Research, University Hospital Ostrava) for their continuous support.

Funding

Ministerstvo Zdravotnictví Ceské Republiky (MHCZ-DRO-FNOs/2015).

Additional information

Funding

Ministerstvo Zdravotnictví Ceské Republiky (MHCZ-DRO-FNOs/2015).