Study of Distribution and Biological Effects of Fullerene C₆₀ after Single and Multiple Intragastrical Administrations to Rats

Abstract

In this study, male rats were intragastrically exposed to fullerene C₆₀ for 1-day and 30-day periods at daily doses of 2000 and 250 mg/kg of body weight, respectively. Fullerene was detected in organs and tissues by HPLC after the extraction from biosamples with toluene. No statistically significant differences in hematological and biochemical parameters of control and treated rats were found after single and multiple administrations. Throughout the observation periods no lethality was observed. At necropsy, no pathomorphological changes in internal organs were recorded. Fullerene was found in stomach, small intestine, liver, lungs, spleen, kidneys, and blood. The amounts of the detected fullerene in comparison to the administered doses are far smaller which is the evidence of its efficient excretion. Hence, it can be assumed that fullerene nanoparticles penetrate from the gastrointestinal tract of rats into the bloodstream and translocate into secondary organs with no pronounced toxic effect in experimental conditions studied.

Keywords:

• fullerene C₆₀
• in vivo
• intragastrical administration
• biodistribution
• biological effects

Additional information

Funding

This study was funded by MARINA project (contract no. 236215) of the EU 7th Framework Program.