Antioxidant ameliorating effects against H₂O₂-induced cytotoxicity in primary endometrial cells

Abstract

Oxidative stress and a disrupted antioxidant system are involved in a variety of pregnancy complications. In the present study, the role of vitamin E (Vit E) and folate as radical scavengers on the GSH homeostasis in stress oxidative induced in rat endometrial cells was investigated. Primary endometrial stromal cell cultures treated with 50 and 200 µM of H₂O₂ and evaluated the cytoprotective effects of Vit E (5 µM) and folate (0.01 µM) in H₂O₂-treated cells for 24 h. Following the exposure of endometrial cells to H₂O₂ alone and in the presence of Vit E and/or folate, cell survival, glutathione peroxidase (GPx) and glutathione reductase activities and the level of reduced glutathione (GSH) were measured. Cell adhesions comprise of cell attachment and spreading on collagen were determined. Flow cytometric analysis using annexin V was used to measure apoptosis. H₂O₂ treatment showed a marked decrease in cell viability, GPx and GR activities and the level of GSH. Although Vit E or folate had some protective effect, combination therapy with Vit E and folate attenuated all the changes due to H₂O₂ toxicity. An increasing number of alive cells was showed in the cells exposed to H₂O₂ (50 µM) accompanied by co-treatment with Vit E and folic acid. The present findings indicate that co-administration of Vit E and folate before and during pregnancy may maintain a viable pregnancy and contribute to its clinical efficacy for the treatment of some idiopathic infertility.

Keywords:

• H₂O₂
• cytotoxicity
• primary
• endometrial cells

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This paper has been supported by Grant Number 5989 from Vice-chancellor for Research Affairs of Shiraz University of Medical Sciences.