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Research Articles

Cleistanthus collinus poisoning affects mitochondrial respiration and induces oxidative stress in the rat kidney

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Pages 561-568 | Received 25 Dec 2018, Accepted 22 May 2019, Published online: 28 Jun 2019
 

Abstract

Cleistanthus collinus is a poisonous shrub used for deliberate self-harm in rural areas of South India and intake of boiled decoction of leaves is a common method of self-harm. Distal renal tubular acidosis (dRTA) is an important clinical symptom observed in C. collinus poisoning, and renal V-ATPases may be potential targets of damage. However, a lack of understanding of molecular mediators involved hampers medical management, which is mainly supportive. We hypothesized that C. collinus poisoning induces renal oxidative stress; probably by inducing mitochondrial uncoupling, which compromises V-ATPase activity to ultimately produce dRTA. This was tested by exposing renal BBMV, kidney cells in culture, and Wistar rats to C. collinus poisoning. Exposure to C. collinus aqueous extract resulted in significant elevations in the lipid peroxidation marker, conjugated dienes, in cell culture and in vivo. A significant decrease in mitochondrial respiratory control ratio was observed in kidneys from C. collinus-treated animals suggesting that mitochondrial oxidative phosphorylation is uncoupled. This was accompanied by significant increase in ADP levels and a decrease in proton pump activity. Thus, these results demonstrate that C. collinus poisoning induces oxidative stress which influences proton pump activity, probably due to feedback inhibition by elevated ADP levels because of mitochondrial dysfunction in the rat kidney.

Acknowledgements

Financial assistance from the Christian Medical College, the Indian Council for Medical Research and Council of Scientific and Industrial Research, Government of India and the South Asian Clinical Toxicology Research Collaboration (SACTRC) is gratefully acknowledged.

Disclosure statement

The authors report no conflicts of interest. The authors are responsible for the content and writing of the paper.

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