Blocking of gastric acid induced histopathological alterations, enhancing of DNA content and proliferation of goblet cells in the acute lung injury mice models by nano-fenugreek oral administration

Abstract

This current study aimed at detecting the potential protective role of nano-fenugreek seed on acute lung injury (ALI) induced by instillation gastric acid in male Swiss albino mice using histological and histochemical studies. Forty animals were grouped as follows: control group, HCl-treated group, low nano-fenugreek + HCl treated group, and high nano-fenugreek + HCl treated group. Pretreatment with nano-fenugreek in animal model of ALI resulted in marked ameliorations of the lung histological lesions and injury induced by HCL instillation in a dose dependent manner. It also caused inhibition in the increase of the DNA content and prevented proliferation of goblet cells induced by HCl instillation alone. In conclusion, pretreatment with Nano-fenugreek prior induction ALI could be suppress the aggregations of inflammatory cells, enhancing of DNA content, and proliferation of goblet cells induced by gastric acid in a dose dependent manner. We suggest that Nano-fenugreek may be useful in combating lung injury.

Keywords:

• Acute lung injury
• nanoparticles
• fenugreek seeds
• DNA content
• goblet cells
• mice

Acknowledgements

We are especially grateful to M. E. El-Naggar, researcher in Textile Research Division, National Research Center, Dokki, Cairo, Egypt for helping in preparation nano-fenugreek. The author gratefully acknowledges the help of the National Organization for Drug Control and Research (NODCAR) departments. The manuscript represents original work that is not being considered elsewhere for publication, in whole or in part.

Disclosure statement

No potential conflict of interest was reported by the authors.