ABSTRACT
Brain injuries, and neurodegenerative diseases can result in significant disability, lowered quality of life, and mortality. Current treatments including autologous nerve grafts are insufficient. This work presents the creation of new biomimetic supramolecular assemblies as a scaffold for potential applications in neural tissue regeneration. It is composed of polyethylene glycol amide core-conjugated with a thiazole-based building block and a peptide sequence (RVYNMGKGKGFCVPRPLVVYR). This peptide sequence is derived from laminin, a basal membrane protein, and a component of the extracellular matrix of neuronal cells. The product formed was allowed to self-assemble and bound to polypyrrole by layer-by-layer assembly to impart conductive properties (Lam-PEG-thiazole-PPy) resulting in the formation of fibrillar assemblies as indicated by electron microscopy studies. Rheological studies demonstrated that incorporation of PPy resulted in higher storage modulus indicative of higher ability to store deformation energy. Culture of rat olfactory-bulb derived neurons with the assemblies demonstrated that the Lam-PEG-thiazole-PPy assemblies promoted cell proliferation and were able to interact favorably with the cells. After 10 days of culture with the assemblies interconnected networks could be visualized. Furthermore, electrical stimulation resulted in a significant increase in axonal outgrowths. Thus, the assemblies presented herein exhibit potential for applications as a novel scaffold for neural tissue engineering.
Acknowledgments
The authors would like to thank Fordham University Chemistry Department for financial support. The authors also thank Dr. K. Fath at the Queens College Core Facility for Imaging, Cell and Molecular Biology for the use of the transmission electron microscope.
Conflicts Of Interest
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish.
Supplementary Material
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