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ABSTRACT
A new method for evaluating the rate constants of chemical reactions has been developed based on the differential photoconversion efficiencies of photochromic FRET acceptors incorporated into molecules on both sides of a chemical equilibrium. In the application of Photochromic Relaxation Kinetics (pcRelKin) to a bimolecular binding reaction, one of the two interacting molecules bears a FRET donor and the second a photochromic acceptor. The close proximity required for FRET is achieved only in the complex. After photoconversion, the system exists in a thermodynamically asymmetric state, which relaxes according to first order kinetics. PcRelKin offers unique advantages over classical relaxation kinetics.
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Acknowledgments
EAJ-E is indebted to the Agencia Nacional de Promoción de la Ciencia y Tecnología (ANPCyT), Fundación Antorchas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Secretaría de Ciencia, Tecnología e Innovación Productiva (SECyT), the German-Argentine DLR-BMBF-SECyT, and the Universidad de Buenos Aires (UBA) for financial support. TMJ was supported by the Max Planck Society. EAJ-E and TMJ were the recipients of a joint grant I/77 897 from the Volkswagen Foundation.