Real-Time Imaging Spectrometry of Tissues for Photodynamic Diagnosis (PDD)

Abstract

A novel imaging spectrometry was demonstrated which can accurately measure the spectrum of whole surface of tissue instead of a single point. Using this apparatus, we obtained the microscope spectrometry image of the hematoxylin-eosin stained tumorous tissue and normal (muscle and blood vessel) mouse tissue was obtained. From the pseudo RGB images of the spectra, tumorous and normal tissues were observed very clearly. The spectra at arbitrary positions could be taken instantly as well as the transmission and absorption spectra, which normalized to the background spectrum. The spectral intensity distribution images were obtained at selected wavelengths to get the area distribution of the stained tumorous and normal tissue clearly. In our experiments, we obtained clear spectrometry imaging from weakly illuminated objects. It took 30 seconds to measure a static image of a sample. Moreover, this apparatus is suitable for real-time measurements of living tissue if using laser illumination, a high-speed CCD camera, a fiber, an endoscope and photosensitizer. In the near future, this novel technique can be used in tumor photodynamic diagnosis.

Keywords:

• hematoxylin-eosin
• imaging spectrometry
• malignant tumor
• microscope
• photodynamic diagnosis
• RGB imaging
• spectral intensity
• spectral intensity distribution images
• tissue screening
• transmittance