Collagen—Dexamethasone and Collagen-D₃ Scaffolds for Bone Tissue Engineering

Abstract

The aim of the study was to test the biocompatibility and osteoinductive potential of collagen with dexamethasone (Dex) and collagen with 1, 25 dihydroxycholecalciferol (D₃) scaffolds on two human osteoblast cell lines, MG63 and hFOB1.19. The cell morphology was examined by SEM, the scaffolds colonization was monitored by fluorescence microscopy, viability by MTT assay and cells differentiation by PCR. The two utilized scaffolds sustained biointegration/proliferation of human osteoblast cells. However, collagen-Dex and collagen-D₃ scaffolds promoted osteogenic activity of MG63 cells incresing the gene expression of osteonectine, osteocalcin and BSP II whereas no osteoinductive effect on hFOB1.19 cells was detected.

Keywords:

• Biocompatibility
• human osteoblast cells
• osteoinduction
• 3D scaffolds

Acknowledgments

This research was financially supported by CNCSIS Postdoctoral Fellowship Programme PNII: Human Resources: PD Nr 134/6.08.2010 and one of the authors (Irina Titorencu) acknowledges the financial support of European Social Fund „Cristofor I. Simionescu” Postdoctoral Fellowship Programme ID POSDRU/89/1.5/S/55216), Sectoral Operational Programme Human Resources Development 2007–2013 (IDPOSDRU/89/1.5/S/55216) and the Romanian Academy.