Substance-P Immobilized Chitosan Nanofibers

Abstract

The surface of biodegradable chitosan nanofibers was functionalized with pristine amine groups and used for immobilization of substance-P, which mediates pain perception and regulates wound healing, inflammation, and angiogenesis. The objectives of this study were to develop substance-P immobilized chitosan nanofibers and to evaluate the biological effects of the immobilized substance-P. Under the same conditions, the amount of substance-P covalently immobilized on chitosan nanofibers was 72.5 ± 16.9 ng compared to 7.3 ± 2.2 ng in passively absorbed samples. Substance-P on chitosan nanofibers released slowly and promoted greater proliferation (∼1.4-fold) of human mesenchymal stem cells than pristine samples after 7 days in culture.

Keywords:

• Chitosan
• substance-P
• wound healing
• electrospinning
• human mesenchymal stem cell
• tissue engineering

Funding

This work was supported by Industrial Strategic Technology Development Program (10035291, Multi-functional medical fiber complex technology) funded by the Ministry of Knowledge Economy (MKE, Korea) and by Nuclear Research Development Program of the Korea Science and Engineering Foundation grant funded by Ministry of Science, ICT, and Future Planning (MSIP, Korea)