Abstract
The APOE ϵ4 gene is a susceptibility gene or risk factor for Alzheimer's disease. The gene codes for a protein which limits the brain's ability to break down amyloid-beta, a protein that is toxic to the brain. In this study, we have prepared magnetic beads - CdSe/ZnS QDs-APOE probe DNA complexes for the detection of APOE DNA. QDs were conjugated to amine- functionalized magnetic beads by EDC/Sulfo-NHS coupling reaction. Thiol group modified single stranded probe oligonucleotides of APOE ϵ2 and APOE ϵ4 were immobilized on the surface of QDs to prepare magnetic bead-QDs-DNA complexes. APOE genes could be detected by fluorescence quenching of QDs by intercalation dye after hybridization. PDMS based micro-fluidic chips were prepared and the bead-QDs-DNA complexes were packed on the channel of the microfluidic chip. The APOE ϵ2 and APOE ϵ4 gene could be detected via fluorescence quenching of QDs on the channel.
Acknowledgment
This research was supported by the GRRC program of Gyeonggi province [(GRRC Gachon 2014-B01)].