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Abstract
L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase.
Abbreviations
| L-ASNase | = | L-asparaginase |
| PEG | = | Poly-ethylene glycol |
| ATPS | = | Aqueous two-phase systems |
| BSA | = | Bovine serum albumin |
| CCC | = | Countercurrent chromatography |
| CPC | = | Centrifugal partition chromatography |
| IEC | = | Ion-exchange chromatography |
| PAA | = | Polyacrylic acid |
| SBP | = | Sugar Beet Pulp |
FUNDING
The authors thank the São Paulo Research Foundation (FAPESP – 2013/08617-7), CAPES, CNPq and Minas Gerais Research Foundation (FAPEMIG) for their support.