Abstract
L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase.
Abbreviations
L-ASNase | = | L-asparaginase |
PEG | = | Poly-ethylene glycol |
ATPS | = | Aqueous two-phase systems |
BSA | = | Bovine serum albumin |
CCC | = | Countercurrent chromatography |
CPC | = | Centrifugal partition chromatography |
IEC | = | Ion-exchange chromatography |
PAA | = | Polyacrylic acid |
SBP | = | Sugar Beet Pulp |
FUNDING
The authors thank the São Paulo Research Foundation (FAPESP – 2013/08617-7), CAPES, CNPq and Minas Gerais Research Foundation (FAPEMIG) for their support.