ABSTRACT
Storage changes of antioxidants in previously developed beverage formulations from Hibiscus sabdarriffa extract, pineapple, oranges and carrots (26.3, 40, 17.2 and 16.5%), respectively was investigated. The blend was filled into bottles and part of it pasteurized at 90°C for 5 min and the other part left unpasteurized. The pasteurized and unpasteurized samples were stored at room and refrigeration temperature for 4 months and were analyzed each month. The pH, vitamin C, carotenoid, color parameters and antioxidants decreased significantly (p˂0.05), while the TTA and microbial loads increased with increase in storage period. Antioxidant activities in all the beverage samples decreased significantly (p˂0.05) after 4 months of storage; however, refrigerated storage temperature maintained higher antioxidants activities. The combination of pasteurization and refrigeration storage retained about 75% vitamin C, 53% carotenoid and 56–79% antioxidants, respectively. Lastly, the study confirmed that the degradation of vitamin C content in the beverages followed the first-order reaction.
Disclosure statement
No potential conflict of interest was reported by the author(s).