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ABSTRACT
The effects of different combinations of plant growth regulators on callus induction and plant regeneration were observed in three Polish monoecious hemp cultivars (Bialobrzeskie, Beniko, Silesia). Callus was induced from different explant sources (fragment of cotyledons, stems, roots) on Daria ind+ medium. There was no considerable difference noticed in callus induction between tested cultivars and explants. However, callus obtained from different genotypes had different ability for organogenesis and plant regeneration. Interaction between tested explant and cultivar has significant effect on the efficiency of plant regeneration: the highest regeneration was observed for cotyledon explant (Beniko), the lowest for stem explants (Silesia).