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ABSTRACT
An experiment was designed to find out the best suited seed protein fraction for identifying the genetic contaminants in seeds of two cotton hybrids viz., CSHH 198 and CSHH 238 where its morphological and genetic differences are meager. The electrophoretic comparison of albumin, globulin, and prolamin seed protein fractions revealed that both albumin and globulin fractions are able to differentiate the hybrids from its male and female parents. The analysis of seed proteins of two cotton hybrid revealed that, albumin fraction produced six different fingerprints with highest discrimination ability than globulin fraction (three). Due to this, albumin fraction showed the added advantage in assessment of genetic purity of CSHH 198 hybrid seed lot that are contaminated with its own parental (selfed female) seeds as well as CSHH 238 cotton hybrid. In contrast, globulin fraction can only be used for identification of off type contaminations due to its own parental seeds and prolamin was unable to differentiate any contaminant. Thus, water soluble albumin protein fraction can be used to differentiate the CSHH 198 and CSHH 238 cotton hybrids and their parental line for both genetic and hybrid purity testing.
用比较蛋白质组分分析对棉花（陆地棉）商业杂交种子纯度检测
实验的目的是要找出最适合的种子蛋白级分用于识别两种杂交棉种子CSHH 198和238 CSHH的遗传污染物，因为它们的形态和遗传差异是微薄的。白蛋白、球蛋白、醇溶蛋白电泳比较，种子蛋白质组分显示，白蛋白和球蛋白组分能够区分杂交的父本和母本。二棉杂种种子蛋白质的分析显示，白蛋白部分产生六个不同的指纹具有最高判别能力相比球蛋白级分（三层）来说。由于这个原因，白蛋白级分表现出在评估CSHH 198杂种种子很多遗传纯度被其自己的亲本的种子混合污染的更多的好处，CSHH 238棉也相同。与此相反，球蛋白级分部分仅可用于由于其自身的亲本种子遗传污染物，而醇溶蛋白的鉴定法是无法区分任何污染物。因此，水溶性白蛋白质级分可用于区分CSHH 198和CSHH 238棉花杂交种及其遗传和杂种纯度亲本检测。