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ABSTRACT
Conventionally, degumming is carried out at 90°C--110°C temperature by boiling the raw silk with Marseilles soap and sodium bicarbonate which eventually requires a lot of water and energy. In this study, degumming of Chinese bivoltine raw silk fibres with alkaline protease produced by Beauveria sp. (MTCC 5184) is studied. Complete degumming was obtained in 45 min with 75 units of enzyme per gram of silk. Degumming was found to be optimal at 50°C and pH 9.0. Scanning electron micrographs showed that the sericin deposits were removed and the obtained fibres were clean, separated, had smooth feel with shine as compared to untreated fibres. Sericin isolated from silk cocoon (by-product which goes waste) was hydrolyzed with the same alkaline protease obtained from Beauveria sp. to get small molecular weight peptides. These peptides can be utilized further for cosmetic, pharmaceutical, and various industrial applications.
摘要
传统上，用马赛皂和碳酸氢钠煮沸生丝，在90°C - 110℃的温度下进行脱胶，最终需要大量的水和能量。在这项研究中，中国生丝脱胶二化性纤维与碱性蛋白酶产生的Beauveria sp.（MTCC 5184）研究。每45克酶解75克酶，得到完全脱胶。在50°C和ph9.0时，脱胶效果最佳。扫描电子显微照片表明，丝胶存款被拆除，所得到的纤维进行清洁，分离，光泽较untreatedfibres有光滑的感觉。从蚕丝的丝胶（副产品分离去渣）用的是同样的碱性蛋白酶从Beauveria sp.得到小分子肽水解。这些肽可进一步用于化妆品、医药和各种工业应用。