Quantifying the Effect of Water Deficit on Cotton Genotypes Using Agro-physiological and Biochemical Parameters

ABSTRACT

To investigate the water-deficit tolerance, 45 cotton genotypes were grown under normal irrigation and water-deficit conditions for 2 years. Genotype × treatment × year interaction was determined for seed cotton yield, number of bolls, boll weight, chlorophyll fluorescence, proline contents, and total soluble proteins. Apart from proline levels, all traits showed a reduction under water-deficit conditions. The mean percentage reduction in seed cotton yield due to water deficit conditions was 52.83% and 42.19%, for number of bolls was 48.44% and 39.30%, for boll weight was reduced by 8.94% and 6.03% in 2013 and 2014 respectively. Reduction in chlorophyll fluorescence was 13.87% and 16.46% while soluble protein levels decreased by 25.57% and 21.09% during 2013 and 2014 respectively. For proline contents went up by 38.68% and 37.70% in 2013 and 2014 respectively. Relatively small changes in these parameters under water-deficit conditions were recorded for varieties FH-155, FH-207, FH-322, FH-329 IUB-13 and IUB-222 making these genotypes best suited for cultivation under water-deficit conditions. The positive correlation of seed cotton yield with proline contents and chlorophyll fluorescence under water-deficit condition indicates that these two traits can be considered to select cotton genotypes at early growth stages.

摘要

为了研究缺水耐受性，45种棉花基因型在正常灌溉和缺水条件下生长，为期两年. 基因型 + 治疗 = 年相互作用被确定为种子棉产量、 薄壳数、 波勒重量、 叶绿素荧光、 脯 氨 酸含量和总可溶性蛋白. 除了亲碱水平外，所有特征在缺水条件下都显示下降. 2013年和2014年，由于缺水状况，籽棉平均减产幅度分别为52.83%和42.19%，棉花产量分别为48.44%和39.30%，其中棉花重量分别下降8.94%和6.03%. 2013年和2014年叶绿素荧光减少13.87%和16.46%，可溶性蛋白水平分别下降25.57%和21.09%. 2013年和2014年，脯 氨 酸含量分别增长了38.68%和37.70%. 在缺水条件下，这些参数的变化相对较小，FH-155、FH-207、FH-322、FH-329 IUB-13和IUB-222的品种，这些基因型最适合在缺水条件下种植. 水缺乏条件下种子棉产量与原蛋白含量和叶绿素荧光的正相关关系表明，这两种特性可考虑在早期生长阶段选择棉花基因型.

KEYWORDS:

• Gossypium hirsutum L.
• genotypes
• water-deficit tolerance
• yield reduction
• proline contents
• total soluble proteins

关键词:

• 高西鸦片平经
• 基因型
• 缺水耐受性
• 产量减少
• 脯 氨 酸内容
• 总可溶性蛋白质

Acknowledgments

The authors would like to thank the Plant Physiology Research Section Ayub Agricultural Research Institute (AARI), Faisalabad, Pakistan for funding and instrumental facility to conduct the physiological and biochemical analysis.