An Effective Degumming Technology for Ramie Fibers Based on Microbial Coculture Strategy

ABSTRACT

Microbial treatment of natural fibers is a preferred alternatives in ramie degumming process as compared to conventional chemical degumming technology with alkaline treatment at high temperature. Usually, it is difficult to degrade the noncellulosic components of ramie bast completely with a single strain in the mono-culturing degumming system. Microbial coculture strategy has been widely used in biotechnology, especially in the degradation of biological materials. In the present study, five strains for ramie degumming were identified as Dickeya dadantii strain DCE-01, Bacillus subtilis strain 1101, Bacillus sp. B6, Bacillus cereus strain B7, and Bacillus cereus strain B8 by sequencing of 16 S rRNA genes. Enzymatic activities characterization, ramie degumming experiments with mono-culturing systems, and the evaluation of strain compatibility showed that group DCE-01/B7 were the most suitable pairs for the construction of bacterial coculturing system. After preliminary optimization of coculturing fermentation condition, the weight loss weight ratio of ramie fibers was 27%, which was increased 13.6% and 8.9% while compared to mono-culturing systems of strain B7 and DCE-01 respectively. These data highlighted the benefit of microbial coculture strategy in the application of ramie degumming process, and indicated a higher-efficiency alternative for the microbial degumming in the textile industry.

 

KEYWORDS:

• Ramie
• fibers
• microbial degumming
• coculture strategy
• enzymatic activity
• fermentation optimization

Acknowledgments

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Natural Science Foundation of China (No. 31700438 and 31871675), Chinese Agricultural Science and Technology Innovation Project (No. ASTIP-IBFC08), Natural Science Foundation of Hunan Province (No. 2019JJ50711 and 2019JJ40331), and China Agriculture Research System for Bast and Leaf Fiber Crops (CARS-16-E22).

Declaration of interest

No potential conflict of interest was reported by the authors.

Supplementary material

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Additional information

Funding

This work was supported by Natural Science Foundation of China [No. 31700438 and 31871675]; Chinese Agricultural Science and Technology Innovation Project [No. ASTIP-IBFC08]; Natural Science Foundation of Hunan Province [No. 2019JJ50711 and 2019JJ40331] and China Agriculture Research System for Bast and Leaf Fiber Crops [CARS-16-E22].