Genetic Diversity and Phylogenetic Analyses of Turkish Cotton (Gossypium hirsutum L.) Lines using ISSR Markers and Chloroplast trnL-F Regions

ABSTRACT

In this study, genetic diversity and phylogenetic relationships of 22 cotton (Gossypium hirsutum L.) lines widely cultivated in Turkey were investigated. For this purpose, 20 ISSR primers were used, and five of which generated amplification products. The polymorphic locus ratio was calculated as 87.21% and a moderate level of genetic diversity was found between the genotypes. Percentage of the highest polymorphic locus was calculated as 12.79% for the Beren line, whereas ADN 98, ADN 710, and Özbek 105 lines showed the lowest value with 0.00%. trnL-F intergenic spacer sequences were used in order to determine phylogenetic relationships, and a comparison was carried out between both sequences of our lines and the sequences obtained from NCBI-GenBank database. The sequences of G. hirsutum, G. hirsutum subsp. latifolium, and G. hirsutum cv. “Hainansijimian” were described as the most similar sequences according to specifications of coverage, similarity, and maximum score ranges. Then, a phylogenetic tree was established by using the sample sequences from the prominent families cultivated, especially from Malvaceae. While the members of Malvaceae family were clustered as one group, the other families were comprised of different groups located in different positions according to their genetic similarities.

摘要

本文研究了在土耳其广泛种植的22个棉花品系的遗传多样性和系统发育关系. 为此，使用了20个ISSR引物，其中5个产生扩增产物. 多态位点比率为87.21%，基因型间存在中等程度的遗传多样性. 贝伦系最高多态位点的百分率为12.79%，而adn98、adn710和zbek105的多态位点比例最低，为0.00%. 利用trnL-F基因间间隔序列确定系统发育关系，并与NCBI-GenBank数据库中获得的序列进行比较. 毛癣菌、毛癣菌亚种的序列. 阔叶草和多毛草. 根据覆盖率、相似度和最大得分区间的规定，将“海南四段”描述为最相似的序列. 然后，利用主要栽培科特别是锦葵科植物的样本序列建立了系统发育树. 锦葵科的成员被聚为一个类群，而其他科则根据它们的遗传相似性分为不同的类群，位于不同的位置.

KEYWORDS:

• Cotton fiber
• cpDNA
• Malvaceae
• molecular breeding
• molecular marker
• phylogeny

关键词:

• 棉纤维
• 锦葵科
• 分子育种
• 分子标记
• 系统发育