Expression Characteristics Analysis of CesA/Csl genes in Flax During the Fast Growing Stage

ABSTRACT

Expression characteristics of predicted CesA/Csl genes of fiber flax variety ‘Diana’ were investigated using RNA-seq data obtained during the fast-growing stage. First, flax stem anatomic analysis revealed differing developmental processes from bottom to top, with middle and lower stem fiber cells exhibiting significantly thickened cell walls. Moreover, stem cellulose/hemicellulose content exhibited positional biases, with a top-to-bottom increase in cellulose content and bottom-to-top increase in hemicellulose content observed. Most CesA/Csl genes were predominantly expressed in stems, roots, and less in leaves. The correlations between the expression levels of CesA/Csl genes and the contents of cellulose and hemicellulose were analyzed. The expression levels of LuCesA4, LuCesA8A and LuCesA8B genes showed significantly positive correlations with cellulose content (P < 0.05), while expression levels of two LuCesA3, three LuCesA6, two LuCslA2, and four LuCslC genes revealed significantly positive correlations with hemicellulose content (P < 0.05). CesA/Csl protein interaction network analysis revealed five transcription factors (TFs) that had high protein–protein interaction (PPI) scores with CesA/Csl proteins: LuNAC10 (Lus10015392), LuNAC12 (Lus10001664), LuNAM (Lus10017915), LuMyb42 (Lus10038913), LuMyb46 (Lus10027369). Therefore, these TFs may play critical regulatory roles in cellulose/hemicellulose synthesis. This study provides a theoretical foundation for investigating flax fiber cell wall synthesis and regulatory mechanisms.

摘要

利用快速生长期获得的RNA-seq数据, 研究了纤维亚麻品种“Diana”预测的CesA/Csl基因的表达特征. 首先, 亚麻茎解剖分析揭示了从下到上的不同发育过程, 中部和下部的纤维细胞显示出明显增厚的细胞壁. 此外, 茎纤维素/半纤维素含量表现出位置差异, 观察到纤维素含量从上到下增加, 半纤维素含量从下到上增加. 大多数CesA/Csl基因主要在茎, 根中表达, 在叶中表达较少. 分析了CesA/Csl基因表达水平与纤维素和半纤维素含量的相关性. LuCesA4, LuCesA8A和LuCesA8B基因的表达水平与纤维素含量呈显著正相关 (P<0.05), 而两个LuCesA3, 三个LuCesA6, 两个Lucesa2和四个LuCslC基因的表达水平与半纤维素含量呈显著正相关 (P<0.05) . CesA/Csl蛋白质相互作用网络分析显示五个转录因子 (TF) 与CesA/Csl蛋白质具有较高的蛋白质相互作用 (PPI) 分数:LuNAC10 (Lus10015392), LuNAC12 (Lus10001664), LuNAM (Lus10017915), LuMyb42 (Lus10038913), LuMyb46 (Lus10027369) . 因此, 这些TF可能在纤维素/半纤维素合成中发挥关键的调节作用. 本研究为亚麻纤维细胞壁的合成和调控机制的研究提供了理论基础.

KEYWORDS:

• Flax
• CesA
• Csl
• expression pattern
• protein–protein interaction network
• secondary cell wall

关键词:

• 亚麻
• CesA; Csl
• 表达模式
• 蛋白质相互作用网络
• 次生细胞壁

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (31701480, 31471546), the China Agriculture Research System of MOF and MARA(CARS-16-S3), Heilongjiang Academy of Agricultural Sciences Foundation(2019KYJL016, 2020FJZX032).