Silk Fibroin Nanoparticles Functionalized with Fibronectin for Release of Vascular Endothelial Growth Factor to Enhance Angiogenesis

ABSTRACT

This work aimed at enhancing the angiogenesis in the presence of silk fibroin nanoparticles functionalized with fibronectin (FN-SF NPs) for controlled release of vascular endothelial growth factor (VEGF). The FN-SF NP samples were synthesized via the desolvation method and then VEGF was encapsulated into them. The physicochemical properties of the VEGF-loaded FN-SF NPs were evaluated by field-emission scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). Results showed the spherical morphology, size of ~90 nm with a polydispersity index of 0.21, and the presence of a characteristic peak in 1063 cm⁻¹ owing to functionalizing between SF NPs and FN. Afterward, the VEGF release from the SF NPs with and without FN functionalization was carried out utilizing enzyme-linked immunosorbent assay (ELISA) and the outcomes revealed that the FN-SF NPs have slower VEGF release than that of unfunctionalized sample with difference of approximately 10%. Eventually, the bioactivity of VEGF released from SF NPs, and FN-SF NPs was assessed using human umbilical vein endothelial cells (HUVECs) culture and the results exhibited a remarkable increase of their proliferation on FN-SF NPs owing to the presence of VEGF even after 10 days.

摘要

本研究的目的是在丝素结合蛋白 (FN-SF NPs) 功能化的丝素纳米颗粒存在下促进血管生成, 以控制血管内皮生长因子 (VEGF) 的释放. 通过去溶剂法合成FN-SF NP样品, 然后将VEGF包裹在其中. 通过场发射扫描电子显微镜 (FE-SEM) 动态光散射 (DLS) 和傅里叶变换红外光谱 (FTIR) 对VEGF负载的FN-SF NP的物理化学性质进行评估. 结果表明, 由于SF-NP和FN之间的功能化, 其球形形貌为~90nm, 多分散指数为0.21, 并且在1063cm-1处存在特征峰. 随后, 利用酶联免疫吸附试验 (ELISA) 对含和不含FN功能化的SF NP的VEGF释放进行了研究, 结果显示, FN-SF NP的VEGF释放速度比未功能化样品慢, 差异约为10%. 最终, 使用人脐静脉内皮细胞 (HUVECs) 培养物评估SF NP和FN-SF NP释放的VEGF的生物活性, 结果显示, 由于VEGF的存在, 即使在10天后, FN-SF NP的增殖也显著增加.

KEYWORDS:

• Angiogenesis
• silk fibroin
• nanoparticle
• fibronectin
• vascular endothelial growth factor
• controlled release
• proliferation

关键词:

• 血管生成
• 丝素蛋白
• 纳米颗粒
• 纳米颗粒
• 血管内皮生长因子
• 控制释放
• 扩散

Disclosure statement

No potential conflict of interest was reported by the authors.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.