Development of Thespesia populnea Doped PVA Electrospun Mat for Biocompatibility Studies

ABSTRACT

Electrospun biocompatible fiber mats were made with Poly Vinyl Alcohol (PVA) using a biocompatible solvent Dimethylsulfoxide (DMSO), because of its compatibility. We have also used the herb “Thespesia populnea” to produce herbal doped electrospun mats. Five samples are produced by varying the percentages of PVA and herbal and the parameters in electrospinning machine. Among those samples, fiber is formed properly when PVA and herbal are taken in the ratio of 5% and 10%, respectively and when Distance, Flow rate, Voltage are set as 15 cm, 0.05 mL/h and 14 kV, respectively. The PVA fiber formation is investigated using a scanning electron microscope (SEM). Fourier Transform Infrared Spectroscopy (FTIR) has been done to confirm the presence of functional group of the herbal and PVA in the mat. Antibacterial testing was done for the samples, and the zone of inhibition was found to be 8–12 mm. Drug release test revealed that the drug from the electrospun mat was better around 70%. In vitro cytotoxicity test was done, and the result obtained was negative since a large amount of DMSO was used for dissolving the herbal. Hence it can be controlled by reducing the usage of DMSO.

摘要

电纺生物相容性纤维垫是以聚乙烯醇 (PVA) 为原料, 采用生物相容性溶剂二甲基亚砜 (DMSO) 制备的. 我们还使用草药“Thespesia popunnea”生产草药掺杂的静电纺垫. 在电纺机上通过改变PVA和草药的比例及参数制备了五种样品. 其中, 当PVA和草药的比例分别为5%和10%, 距离、流速、电压分别设置为15cm、0.05mL/h和14 kV时, 纤维形成良好. 利用扫描电子显微镜 (SEM) 研究了PVA纤维的形成. 傅里叶变换红外光谱 (FTIR) 已被用于确认垫子中存在草药和PVA的官能团. 对样品进行了抗菌测试, 发现抑制区为8-12mm. 药物释放试验表明, 从静电纺丝垫中得到的药物在70%左右更好. 进行了体外细胞毒性试验, 结果为阴性, 因为大量二甲基亚砜用于溶解草药. 因此, 可以通过减少二甲基亚砜的使用来控制它.

KEYWORDS:

• Biocompatible
• PVA
• DMSO
• Thespesia populnea
• SEM
• FTIR
• In vitro cytotoxicity test

关键词:

• 生物相容性
• 体外细胞毒性试验

Disclosure statement

No potential conflict of interest was reported by the author(s).

Highlights of the Research article

• Among those samples, fiber is formed properly when PVA and herbal are taken in the ratio of 5% and 10%, respectively and when Distance, Flow rate, and Voltage are set as 15 cm, 0.05 mL/h, and 14 kV, respectively.

• Antibacterial testing was done for the samples and the zone of inhibition was found to be 8-12 mm.

• Drug release test revealed that the drug from the electrospun mat was better around 70%.

• In vitro cytotoxicity test was done, and the result obtained was negative since large amount of DMSO was used for dissolving the herbal. Hence it can be controlled by reducing the usage of DMSO.

• The cytotoxicity results from the figure, inferred that cell growth does not get disturbed and extensively affected by the herbal Thespesia populnea.

• The novelty of the study is to study the Antibacterial activity and drug release characteristics of Thespesia populnea through biocompatibility testing unlike the regular wound dressing testing since compatibility is the key aspect that has been determined and the results are compared between the cast films and extract incorporated electrospun fiber mats.