Construction of an evenly-distributed genetic map using contig-tag-SNPs for quantitative trait loci (QTL) analysis of fiber-related traits in kenaf (Hibiscus cannabinus L.)

ABSTRACT

Kenaf is one of the most important natural fiber crops worldwide, which aims at harvesting bast fiber. Mining QTL loci of fiber yield and quality traits will facilitate fiber improvement and molecular marker-assisted breeding in kenaf. In this study, Fuhong 952 and Zanyin No. 1 were used as parents to construct two mapping populations, F₂ and F_2:3, and an evenly distributed genetic linkage map was constructed by re-sequencing. The map contains 2512 contig-tag-SNP markers, and 18 linkage groups with a total length of 1287.63 cM and an average distance of 0.51 cM. Totally, 32 and 28 QTLs were detected in the F₂ and F_2:3 populations, respectively. Through Blast searching against the reference genome using the sequences of flanking molecular markers linked to QTLs, 374 candidate genes related to cell wall formation and photoperiod regulating flowering were found in these loci, including cellulose synthase-like genes, MYB genes, and Agamous-like genes. These findings could lay a foundation for the improvement of fiber-related traits and gene cloning in kenaf.

摘要

红麻是世界上最重要的天然纤维作物之一，旨在收获韧皮纤维。挖掘红麻纤维产量和品质性状的QTL位点将有助于红麻纤维相关性状改良和分子标记辅助育种。本研究以福红952和赞引1号为亲本, 构建了两个作图群体F₂和F_2:3, 并通过重测序构建了均匀分布的遗传连锁图谱。该图谱包含2512个contig标签SNP标记和18个连锁群，总长度为1287.63 厘摩，平均距离为0.51 厘摩。在F₂和F_2:3群体中分别检测到32个和28个QTLs。通过使用与QTL连锁的侧翼分子标记序列对参考基因组进行Blast比对，在这些基因座中发现了374个与细胞壁形成和光周期调节开花相关的候选基因， 包括类纤维素合成酶基因、MYB基因和类Agamous基因。这些发现为红麻纤维相关性状的改良和基因克隆奠定了基础。

KEYWORDS:

• Kenaf
• fiber yield
• genetic map
• QTL mapping

关键词:

• 红麻
• 纤维产量
• 遗传图谱
• QTL定位

Disclosure statement

No potential conflict of interest was reported by the author(s).

Consent to participate

All authors read and approved the final manuscript.

Consent to publication

All authors approved the published manuscript.

Author’s contribution

LZ has designed and supervised experiments. YX and XW helped to prepare the reagents and materials. YX and SC carried out the data analysis and wrote the paper. TL, JX, and XW participated in the editing and revision of the manuscript. JQ and LZ contributed to germplasm and techniques. All authors read and approved the final manuscript.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/15440478.2022.2072443

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [31972968]; the China Agriculture Research System of MOF and MARA (CARS-16).