Schizophyllum commune-derived Chitin Glucan Complex Wound Dressing: Antibacterial Activity and Wound Healing Properties in a Second Degree Burn Animal Model

ABSTRACT

The chitin-glucan complex (CGC) consists of β-glucan and chitin with covalant bonds. CGC with biodegradable, biocompatible, antioxidant, and antibacterial properties is a powerful agent in wound healing. Schizophyllum commune is a source of these metabolite. This study aims to optimize the preparation of CGC nanofibers by electrospinning. Optimal conditions were selected for fabricating nanofibers (10% PVA solution, 2% CGC solution, 0.29 g gelatin, and 2.3 mL of acetic acid) with 17 kV, 900 rpm, and a feed rate of 0.3 mL/min. SEM showed uniform nanofibers with a diameter of 210 ± 71 nm and 56% porosity. FTIR investigates possible interactions between components. CGC/PVA/Gelatin nanofibers are hydrophilic. The tensile strength and Young’s modulus reached 4.223, 2.2092 Mpa, respectively. CGC/PVA/Gelatin nanofibers inhibit the growth of E. coli and S. aureus by 25% and 78% after 24 hours, respectively. These nanofibers are nontoxic to fibroblast cells and improve their proliferation and adhesion. CGC/PVA/Gelatin nanofibers were used to cover grade 2 burn wounds in male Wistar rats. Morphometric results showed that the wound healing rate is 86% in the nanofibers treated group (p< 0.05) and 58% and 49% in the positive and negative control groups. S. commune CGC nanofibers can be used as wound dressings.

Gragphical Abtract

摘要

几丁质-葡聚糖复合物(CGC)由β-葡聚糖和具有共价键的几丁质组成. CGC具有生物可降解、生物相容性、抗氧化和抗菌特性,是一种强有力的伤口愈合剂. 裂褶菌群是这些代谢物的来源. 本研究旨在优化静电纺丝制备CGC纳米纤维的工艺. 选择了制备纳米纤维的最佳条件(10%PVA溶液、2%CGC溶液、0.29 g明胶和2.3mL乙酸),电压为17 kV,转速为900rpm,进给速度为0.3mL/min. SEM显示纳米纤维直径为210±71 nm,孔隙率为56%. FTIR研究组分之间可能的相互作用. CGC/PVA/明胶纳米纤维具有亲水性. 拉伸强度和杨氏模量分别达到4.223、2.2092mpa. CGC/PVA/明胶纳米纤维对大肠杆菌和金黄色葡萄球菌的生长抑制率分别为25%和78%. 这些纳米纤维对成纤维细胞无毒,可促进其增殖和粘附. 用CGC/PVA/明胶纳米纤维覆盖雄性Wistar大鼠2级烧伤创面. 形态计量学结果显示,纳米纤维治疗组创面愈合率为86%(p<0.05),阳性对照组和阴性对照组分别为58%和49%. S、 commune CGC纳米纤维可用作伤口敷料.

KEYWORDS:

• Schizophyllum commune
• chitin-glucan complex
• nanofibers
• antibacterial
• wound healing

关键词:

• 裂褶菌公社
• 几丁质-葡聚糖复合物
• 纳米纤维
• 抗菌
• 伤口愈合

Acknowledgments

The authors would like to thank all people, who helped us during the experimental work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Highlights

• S. commune CGC/PVA/Gelatin nanofibers were prepared by electrospinning.

• CGC/PVA/Gelatin nanofibers inhibited the growth of E. coli and S. aureus.

• CGC/PVA/Gelatin nanofibers were non-toxic and improved fibroblasts proliferation.

• CGC/PVA/Gelatin nanofibers healed grade 2 burn wounds about 86% after 14 days.