Determining the Genetic Difference of Some World Cotton Genotypes Using iPbs (Inter-Primer Binding Sequences) Retrotransposon Markers

ABSTRACT

This research was carried out with the aim of revealing the genetic differences of 30 cotton varieties from 14 countries using the iPBS-retrotransposon marker method. Genetic differences were determined by using iPBS marker method in PCR for 30 cotton varieties. According to the genetic distance matrix obtained in the NTSYS-pc 2.10e program, the closest genotypes were determined to be AzGR-11468 (Azerbaijan) and Maydos Yerlisi (India), and the similarity value between them was found to be 0.0318. The most distant genotypes were determined to be Hill Cotton 3 (Bangladesh) and Shazbaz (Pakistan), and the similarity value between them was found to be 0.7572. When the dendrogram generated based on the UPGMA method was examined.30 cotton varieties were divided into 2 clades. According to the PCA made in the JMP program, the graphic results and eigen values obtained on the two-dimensional plane also supported these results. The first 3 eigen values explained 85% of the total variance in the population. Overall results suggest that Hill Cotton 3 (Bangladesh) (Gossypium arboreum) and Shazbaz (Pakistan) (Gossypium hirsutum) cultivars, which are the most distant relatives among 30 cotton genotypes, can be used as breeding cultivars in future cotton breeding studies.

摘要

本研究旨在利用iPBS逆转录转座子标记法揭示来自14个国家的30个棉花品种的遗传差异. 采用iPBS标记法对30个棉花品种进行了PCR检测. 根据NTSYS pc 2.10e程序中获得的遗传距离矩阵，最接近的基因型被确定为AzGR -11468（阿塞拜疆）和Maydos Yerlisi（印度），它们之间的相似值为0.0318. 最远的基因型被确定为Hill Cotton 3（孟加拉国）和Shazbaz（巴基斯坦），它们之间的相似值为0.7572. 当检查基于UPGMA方法生成的树状图时. 30个棉花品种分为2个分支. 根据JMP程序中的主成分分析，在二维平面上获得的图形结果和特征值也支持这些结果. 前3个特征值解释了总体总方差的85%. 总体结果表明，Hill Cotton 3（孟加拉国）和Shazbaz（巴基斯坦）是30个棉花基因型中最远亲的棉花品种，可作为未来棉花育种研究的育种品种.

KEYWORDS:

• Malvaceae
• cotton
• iPBS
• marker
• genetic
• world

关键词:

• 锦葵科
• 棉
• 标记
• 遗传的
• 世界

Disclosure statement

No potential conflict of interest was reported by the author(s).