The YAP1-TEAD axis promotes autophagy against intracellular Staphylococcus aureus in vitro

ABSTRACT

Previously considered as an exclusive extracellular bacterium, Staphylococcus aureus has been shown to be able to invade many cells in vitro and in humans. Once inside the host cell, both cytosolic and endosome-associated S. aureus strongly induce macroautophagy/autophagy. Whether autophagy fosters S. aureus intracellular survival or clearance remains unclear. The YAP1-TEAD axis regulates the expression of target genes controlling the cell fate (e.g., proliferation, migration, cell cycle …). Growing evidence indicates that YAP1-TEAD also regulates autophagy and lysosomal pathways. Recently we showed that the YAP1-TEAD axis promotes autophagy and lysosome biogenesis to restrict S. aureus intracellular replication. We also discovered that the C3 exoenzyme-like EDIN-B toxin produced by the pathogenic S. aureus ST80 strain inhibits YAP1 nuclear translocation resulting in a strong increase of intracellular S. aureus burden.

KEYWORDS:

• Autophagy
• C3 exoenzyme
• cell-autonomous immunity
• EDIN
• host response genes
• inflammation
• lysosome
• staphylococcus aureus
• YAP

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants from the Foundation for the University Jean Monnet of St-Etienne AAP Fonds d’Amorçage Recherche 2023 (AAP Fonds d’Amorçage Recherche 2023 - POV) and the FINOVI foundation (#AO13 - POV) under the aegis of the Foundation for the University of Lyon.