Identification and antibiotic resistance of Lactobacilli isolated from raw cow’s milk of Oujda City (Morocco)

ABSTRACT

In this study, a Lactobacillus strain was isolated from raw cow milk. It was tentatively identified at the species level according to its carbohydrate fermentation profile and phenotype characteristics as Lactobacillus paracasei KSNM. The susceptibility of the identified Lactobacillus to 23 antibiotics was performed using the disc diffusion testing method. Inhibition zone diameters were measured and expressed in terms of sensitive, intermediate or resistant. KSNM strain was sensitive toward; amoxicillin, ampicillin, amoxyclav (amoxicillin + clavulanic acid), tetracycline, macrolides, sulfamides/trimethoprim, and rifampicin. But, it expressed intermediate sensitivity toward; cefamandole, cefaclor, and cephalothin. However, it was resistant to penicillin G, aminoglycosides, quinolones, ciprofloxacin, lincomycin, nitroxolin, and a large number of cephalosporins, which included cefoxitin, cefotaxime, and ceftriaxone.

KEYWORDS:

• Antibiotic sensitivity
• isolation
• lactic acid bacteria
• Lactobacilli
• raw milk