Screening of thermotolerant yeast by low-energy ion implantation for cellulosic ethanol fermentation

ABSTRACT

One thermotolerant mutant, ST-1559, was selected after mutagenizing with ion beam implantation. The results showed that ST-1559 could grow well under 45°C,and its ethanol yield reached 109.46 g/L when the glucose content was 250 g/L in the fermentation medium. When corn straw which was pretreated by NaOH was used as substrate in fermentation experiment under 45°C, it produced 13.48 g/L ethanol, and 82.17% of the cellulose was achieved. These results suggest that ST-1559 had advantage in simultaneous saccharification and fermentation.

KEYWORDS:

• Corn straw
• fermentation
• low-energy ion implantation
• mutant
• thermotolerant yeast

Additional information

Funding

The authors are grateful for the financial support provided by the National Natural Science Foundation of China (Grant No. 31770636) and Basic Scientific Research Project of Jiangsu Province Biomass Energy and Material Laboratory (JSBEM-S-2017011).