Second generation bioethanol production from hemicellulolytic hydrolyzate of apple pomace by Pichia stipitis

ABSTRACT

Investigation of novel feedstocks and microorganisms is very important for bioethanol production. Bioethanol production from hemicellulolytic hydrolyzate of the apple pomace was aimed in this study. Effects of some critical parameters such as biomass loading (5, 10, 15% w/v), supplements (nitrogen and mineral sources), and enzymatic hydrolysis on bioethanol production were optimized. The highest theoretical ethanol yield was observed as 84.1%, and Pichia stipitis produced 14.36 g/L bioethanol in the presence of the 10% (w/v) apple pomace loading. This is the first report about the enzymatic hydrolysis of the liquid fraction of apple pomace for bioethanol production by Pichia stipitis.

KEYWORDS:

• Hemicellulolytic hydrolyzate
• apple pomace
• pichia stipitis
• enzymatic hydrolysis
• bioethanol

Disclosure statement

The authors reported no potential conflict of interest.

Additional information

Funding

This work was supported by the Research Foundation of Ankara University (Project Number: 19L0430008), and Aybüke KUT was awarded a M.S. scholarship from TUBITAK;Ankara Universitesi [19L0430008];

Notes on contributors

Aybüke Kut

Aybüke Kut completed her undergraduate in the Ankara University, Science Faculty, Biology Department. She is carrying out her M.S studies in the same institution. Her research area is mainly focused on bioethanol production from lignocellulosic feedstocks. She has awarded for a M.S. scholarship from Scientific and Technological Research Council of Turkey (TUBITAK).

Ekin Demiray

Ekin Demiray completed his undergraduate and Ph.D. degrees at Ankara University. He participated in postdoctoral studies at Ankara University. His research interests are biofuels.

Sevgi Ertuğrul Karatay

Sevgi Ertuğrul Karatay completed her undergraduate and Ph.D. degrees at Ankara University. She participated in postdoctoral studies at Ankara University and Ghent University (Belgium). She is currently a Professor in the biology department of the Faculty of Science at Ankara University, Turkey. Her research interests are biofuels, wastewater treatment technologies, environmental biotechnology and microbiology.

Gönül Dönmez

Gönül Dönmez completed her undergraduate, Ph.D., and postdoctoral studies at Ankara University, Turkey. She is currently a Professor in the biology department of the Faculty of Science at Ankara University, where she has been a faculty member since 1987. Her research interests are biofuels, biosorption, biodegradation, bioaccumulation, environmental biotechnology, wastewater treatment, microbiology and algae technology