Optimization of preparation conditions of sulfate-reducing bacteria (SRB) immobilization particles strengthened by maifanite and its treatment of wastewater containing Mn²⁺

ABSTRACT

According to the characteristics of acid mine drainage (AMD) solution, such as extremely low pH, rich SO₄²⁻, and Mn²⁺ pollution ions. In this study, maifanite, bagasse, and sulfate-reducing bacteria (SRB) were used as research objects based on microbial immobilization technology. Response surface methodology was used coupled with the Box–Behnken design. The type, dosage, and particle size of maifanite were used as independent variables. The pH, oxidation-reduction potential, total Fe (TFe) removal efficiency, Mn²⁺ removal efficiency, SO₄²⁻ removal efficiency, and COD release amount of maifanite-reinforced SRB-immobilized particles were used as response values to optimize the preparation parameters of maifanite-reinforced immobilized particles, and the adsorption characteristics were analyzed. The results showed that the optimal conditions for the preparation of maifanite-reinforced SRB-immobilized particles were as follows: the type, dosage, and particle size of maifanite were Dengfeng maifanite, 0.1 g and 200–300 mesh, respectively. The surface pores of AMD were reduced and FeS, MnS, Fe(OH)₃, Mn(OH)₂ appeared after treated with maifanite, bagasse, and SRB. The reduction process of SO₄^2- by maifanite-enhanced SRB-immobilized particles conformed to the first-order kinetics, and the adsorption process of Mn²⁺ conformed to the McKay pseudo-second-order kinetic model and the Freundlich isothermal adsorption model.

KEYWORDS:

• Acid mine drainage
• maifanite
• sulfate-reducing bacteria
• immobilized particles
• optimization

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was supported by the National Natural Science Foundation of China (41672247), discipline innovation team of Liaoning Technical University (<#AWARD-ID;>LNTU20TD-21</#AWARD-ID;>).

Notes on contributors

Sihang Bao

Sihang Bao received the B.S. degree in Water Science and Engineering from Shenyang University, Shenyang, China, in 2017 and the M.S. degree in Water Engineering from Universiti Putra Malaysia , Kuala Lumpur, Malaysia in 2020. She is currently working toward the Ph.D. degree in Mineral Engineeringwith the College of Mining, Liaoning Technical University, Fuxin, China. Her research interests include Waste water treatment and waste water recycling.

Junzhen Di

Junzhen Di received the B.S. degree , M.S. degree and Ph.D. degree from Liaoning Technical University, Fuxin, China. His research interests include Wastewater treatment, wastewater recovery and reuse and water quality and water treatment technology.

Yanrong Dong

Yanrong Dong received the B.S. degree , M.S. degree and Ph.D. degree from Liaoning Technical University, Fuxin, China. Her research interests include microbiological treatment and microbial treatment of wastewater.

Xianjun Wang

Xianjun Wang received the B.S. degree and M.S. degree from Liaoning Technical University, Fuxin, China. He is currently working toward the Ph.D. degree in the College of Civil Engineering, Liaoning Technical University, Fuxin, China. His research interests include Waste water treatment and waste water recycling.

Lindan Xue

Lindan Xue received the B.S. degree and M.S. degree from Liaoning Technical University, Fuxin, China. Her research interests include Water treatment technology and waste water recovery and reuse.

Juan Sun

Juan Sun received the B.S. degree and M.S. degree from Liaoning Technical University, Fuxin, China. Her research interests include Treatment of mine wastewater and water treatment technology.