100
Views
0
CrossRef citations to date
0
Altmetric
Research Articles

Vanadium inhalation effects on the corneal ciliary neurotrophic factor (CNTF): study in a murine model

, ORCID Icon, ORCID Icon & ORCID Icon
Pages 49-54 | Received 28 Sep 2022, Accepted 02 Feb 2023, Published online: 14 Feb 2023
 

Abstract

Purpose

Air pollution is a public health problem caused by predatory human activities and the indiscriminate burning of fossil fuels that liberate particulate matter (PM) into the atmosphere. Vanadium (V) adheres to them and reaches the bloodstream and different organs such as the eye when inhaled. Another way to reach the eye is by direct contact, and the cornea is the first layer exposed. Ciliary neurotrophic factor (CNTF) is secreted by the corneal nerves and some of its functions include self-renewal maintenance and wound healing by the activation of STAT3. Previous reports from our group indicate the activation of STAT3 after the inhalation of V, adhered to PM.

Objective

To analyse the effect of V inhalation in the expression of CNTF. Method: CD-1 male mice were exposed for 4 and 8 weeks to V inhalation. The eyes were removed, and the corneas were processed for immunohistochemistry for CNTF and analysed by densitometry. The same slides were used to evaluate histological modifications and to measure the corneas’ anterior epithelial and endothelial thickness.

Results

A decrease in CNTF expression in the anterior epithelium in the 8th week, as well as an increase in the endothelial and corneal thickness and disarray of all the layers of the anterior epithelium.

Conclusion

V inhalation disturbs the architecture of the cornea and modifies the presence of CNTF which might modify the renewal of the corneas after exposure to PM air pollution.

Acknowledgements

The authors thank Armando Zepeda-Rodríguez and Francisco Pasos-Nájera Departamento de Biología Celular y Tisular, Facultad de Medicina, UNAM, for artwork with the figures. We also appreciate the technical assistance of Ismael Torres-Saldaña, a member of the vivarium staff of the Facultad de Medicina, UNAM. Alejandra Núñez-Fortoul edited the English of the final version of the manuscript.

Declaration of interest

The authors declare that there are no conflict of interest.

Additional information

Funding

This research was supported by the Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México IN200221.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,568.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.