Abstract
In the present work, chicken (IgY) and rabbit (IgG) antibodies were generated against the venoms of Daboia russelii and Echis carinatus snakes and efficacy was compared with commercial antivenom. Antivenom antibodies were purified and evaluated by ELISA, SDS-PAGE, Western Blot. All antivenoms neutralized a challenge dose of 3LD50. Each ml of IgY, IgG and commercial antivenom neutralized 0.3, 0.34, and 0.6 mg of E. carinatus venom and 0.2, 0.22 and 0.57 mg D. russelii venom respectively. The present study suggests the generated antivenoms as effective in neutralizing the venoms injected, implying the scope of IgY antivenoms for further screening and evaluation.
Acknowledgments
The authors would like to thank Department of Microbiology, PSG College of Arts and Science and Department of Molecular Pharmacology, PSG College of Pharmacy Coimbatore for providing all necessary facilities to carry out this research work. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at the King Saud University, Riyadh, Saudi Arabia for funding this Research Group Project (No. RGP-289)
Ethical approval
All experiments involving the use of animals were ethically approved by the Institutional Animal Ethical Committee (IAEC) at PSG Institution of Medical Science and Research, Coimbatore with proposal approval No. 385/2018/IAEC and 402/2018/IAEC.
Disclosure statement
No potential conflict of interest was reported by the author(s).