Lophodermium macci sp. nov., a new species on senesced foliage of five-needle pines

Abstract

The new species Lophodermium macci is described. It is similar in its morphology, habitat, geographic range and ecology to L. pini-excelsae, L. staleyi and L. nitens and often is misidentified as L. pinastri on Pinus strobus in herbaria. A modified technique was used to extract DNA from minute ascomata on herbarium specimens, and new primers were made to amplify the damaged DNA from these specimens. It provides added evidence to separate L. macci from L. pini-excelsae, its closest morphological taxon.

• DNA
• herbarium specimens
• ITS
• Lophodermium
• L. pinastri
• L. pini-excelsae
• Pinus strobus
• systematics

The authors thank Marie Simard for specimen preparation and thin sections, Jean-Philippe Doyon for the black and white drawings, Franck Stefani for helping with the design of the primers and bayesian analysis and the curators of DAOM, IMI and QFB for the loans of specimens.