The response of ectomycorrhizal fungal inoculum to long-term increases in nitrogen supply

Abstract

The inoculum of ectomycorrhizal (EM) fungi was examined in a 16 y long nitrogen fertilization experiment maintained in a temperate oak savanna. To measure EM fungal inoculum, bur oak seedlings were grown in three types of bioassays: (i) intact soil cores that measure inoculum such as spores, mycelia and mycorrhizal roots; (ii) resistant propagule bioassays that measure inoculum types resistant to soil drying; and (iii) previously mycorrhizal root bioassays that measure the ability of EM fungi to colonize new roots from mycorrhizal roots. Colonization of bur oak seedlings was characterized by morphotyping and where necessary by restriction analysis and internal transcribed spacer (ITS) sequencing. Fourteen morphotypes were found in intact soil core bioassays with species of Cortinarius, Cenococcum and Russula abundant. Five morphotypes were found in resistant propagule bioassays with Cenococcum, a thelephoroid morphotype and a Wilcoxina-like ascomycete abundant and frequent. In intact soil core bioassays total percent root colonization and number of morphotypes were not affected by N supply in 2000 and 2001. However the composition of EM fungi colonizing oak seedling roots was different with increased N supply such that Russula spp. (primarily Russula aff. amoenolens) were most abundant at the highest level of N supply. Dominant Russula spp. did not colonize any roots in resistant propagule bioassays but did colonize oak seedling roots from previously mycorrhizal roots. Results suggest that in this savanna N supply can influence the kinds of inoculum propagules present and thereby might affect the dynamics of ectomycorrhizal communities by differentially influencing reproductive and colonization strategies.

• Cenococcum
• Cortinarius
• ectomycorrhizal fungi
• inoculum bioassay
• nitrogen
• oak seedlings
• Russula

The assistance and suggestions of N. Blumm, D. Marks, L. Marlowe, A. Silver, S. Stundins, the Charvat Lab, Mc-Laughlin Lab, Morrow Lab and Reich Lab at the University of Minnesota is greatly appreciated. D. McLaughlin, I. Dickie, B. Roy and two anonymous reviewers provided valuable comments on drafts of this manuscript. Financing for this project was provided by the University of Minnesota’s Plant Biological Sciences Graduate Program, Graduate School, the Dayton-Wilkie Fund and the Minnesota Agricultural Experiment Station.