In vitro effects of cobalt and chromium nanoparticles on human platelet function

Abstract

Nanoparticles (NPs) are released from orthopedic and neurosurgical prostheses and can interact with a number of blood components once in the bloodstream. Potential toxic effects of Co and Cr NPs on blood platelets have not been thoroughly investigated. The aim of this study was to analyze the effect of Co and Cr NPs on platelet function in vitro. The ability of the tested NPs to induce platelet activation and aggregation was measured using light transmission aggregometry, flow cytometry, and quartz crystal balance with dissipation (QCM-D). This was confirmed by transmission electron microscopy (TEM), scanning electron microscopy, and optical and immunofluorescence microscopy. Perfusion of QCM-D sensor crystals with platelet-rich-plasma in the presence of Co 28 nm, CoO 50 nm, Co₂O₃ 50 nm, Co₃O₄ 30–50nm, Cr 35–45nm, Cr₂O₃ 60 nm NPs (0.5–5.0 µg/mL) resulted in significant changes in frequency and dissipation, indicating that these NPs caused platelet microaggregation. Transmission electron microscopy also revealed that Cr NPs led to platelet swelling and lysis. Our study shows that both Co and Cr NPs affect platelet function in vitro with two distinct mechanisms. While Co NPs result in standard platelet aggregation, Cr NPs cause both platelet aggregation and decreased platelet membrane integrity and lysis. Based on these findings, monitoring serum NP levels and platelet-mediated hemostasis can be advised in patients with metal-on-metal Co–Cr prostheses.

Keywords:

• Nanoparticles
• cobalt
• chromium
• platelet
• total hip arthroplasty

Acknowledgements

The authors would like to thank Prof. Maria Jose Santos-Martinez for her help with QCM-D experiments, Dr Karolina Kaczmarczyk-Sekuła for her help with TEM imaging, and Dr Katarzyna Berent for her help with SEM imaging.

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

The present work was financially supported by the National Science Center Poland (SONATA program no. [UMO-2015/19/D/NZ5/02189]).