Characterization of the antigenic specificity of soybean protein β-conglycinin and its effects on growth and immune function in rats

Abstract

The objective of the present study was to characterize the antigenic specificity of purified soybean β-conglycinin and to investigate its effects on the growth and immune responses of rats. Thirty-two Brown Norway rats, 3 weeks of age, were randomly allotted to one of four treatments and individually fed casein-cornstarch based diets. Rats were sensitised by means of intragastric gavage with purified β-conglycinin (0, 5, 10 or 20 mg protein/ml in phosphate buffered saline at pH 7.4) on day 0, 7, 14, and 21 (1 ml/animal). On day 28, rats received a double dose of β-conglycinin. Blood was obtained at weekly intervals after initiation of challenge. Growth declined linearly with increasing the concentration of soybean β-conglycinin (p < 0.05). Both the total IgE and β-conglycinin-specific IgE levels in serum increased while passive cutaneous anaphylactic reactions were induced in the rats. Lymphocyte proliferation response to concanavalin A in plasma and spleen was increased linearly with increased levels of soybean (p < 0.01) β-conglycinin. The percentage of CD4⁺ lymphocyte subset linearly increased (p < 0.001). As a result, the concentrations of cytokines in plasma and spleen, including interleukin-4 (p < 0.01), interleukin-5 (p < 0.01), and tumour necrosis factor-α (p < 0.01) increased linearly with increasing level of purified β-conglycinin. Our results indicate that purified β-conglycinin possesses intrinsic immune-stimulating capacity and can induce an allergic reaction. Therefore, dietary soybean β-conglycinin has negative effects on growth and both cell-mediated and humoral immune function in rats.

Keywords:

• β-conglycinin
• antigenic specificity
• IgE
• immune response
• growth
• rat

Acknowledgements

The investigation was financially supported by the National Natural Science Foundation of P. R. China (No. 30430520).