Inulin and probiotics in newly weaned piglets: effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology

Abstract

The study aimed at determining the effect of inulin and/or a multispecies probiotic formulation on gastrointestinal tract (GIT) morphology, immunological and haematological parameters. Forty-eight newly weaned piglets were assigned to four feeding groups, receiving a standard basal diet (control), supplemented with 0.4% inulin, probiotics (1 · 10⁹ CFU/kg as fed, enterococci, lactobacilli, bifidobacteria) or a combination of both (synbiotic). After four weeks of ad libitum feeding piglets were slaughtered and intestinal tissue samples were obtained for histometry. Additional tissue samples of the GIT, mesenteric lymph nodes, blood, liver and spleen were taken for mRNA expression analysis of cell turnover (CDK4, caspase3, IGF I), transcription factor NFκB and inflammatory marker genes (TNFα, TGFβ). Changes in histometry occurred predominantly in the small intestine, showing higher jejunal villi when probiotics were administered alone (p < 0.10). Inulin decreased the number of acidic goblet cells in jejunal villi (p < 0.05), whereas probiotics increased neutral goblet cells in ileal villi (p < 0.05). Though inflammatory marker genes were uninfluenced by treatment in the proximal GIT, the colon showed downregulations induced by inulin (TNFα: p < 0.10, TGFβ: p < 0.05). Gene expression of CDK4 was upregulated in the jejunum and of TGFβ in the mesenteric lymph nodes in the probiotic groups. Interestingly, the probiotic group alone exhibited upregulations in cell turnover marker genes in the colon and blood. Furthermore, for numerous parameters, inulin and probiotics led to no synergistic but antagonistic interactions.

Keywords:

• gene expression
• haematology
• morphology
• intestine
• inulin
• piglets
• probiotics

Acknowledgements

The authors are very grateful to the Landwirtschaftliche Fachschule Hatzendorf for providing facilities for the feeding trial and to Herbert Telser for animal care. Moreover, the authors wish to thank the co-workers of the involved divisions for aid with laboratory analyses, and Sabine Masching as well as Dr Gerd Schatzmayr (Biomin GmbH, Herzogenburg, Austria) for cooperation and providing feed.